Demultiplex: FASTA/FASTQ demultiplexerΒΆ

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Versatile NGS demultiplexer with the following features:

  • Support for FASTA and FASTQ files.

  • Support for gzip and bzip2 compressed files.

  • Support for multiple reads per fragment, e.g., paired-end.

  • Handles barcodes in the header and in the reads.

  • Handles barcodes at unknown locations in reads (e.g., PacBio or Nanopore barcodes).

  • Support for selection of part of a barcode.

  • Allows for mismatches, insertions and deletions.

  • Barcode guessing by frequency or fixed amount.

  • Handles large numbers (over one million) of barcodes.

Please see ReadTheDocs for the latest documentation.