Demultiplex: FASTA/FASTQ demultiplexerΒΆ

Versatile NGS demultiplexer with the following features:

  • Support for FASTA and FASTQ files.
  • Support for gzip and bzip2 compressed files.
  • Support for multiple reads per fragment, e.g., paired-end.
  • Handles barcodes in the header and in the reads.
  • Handles barcodes at unknown locations in reads (e.g., PacBio or Nanopore barcodes).
  • Support for selection of part of a barcode.
  • Allows for mismatches, insertions and deletions.
  • Barcode guessing by frequency or fixed amount.
  • Handles large numbers (over one million) of barcodes.

Please see ReadTheDocs for the latest documentation.